Single-Cell Analysis: Microsystems for Single-Cell Analysis (Adv. Biosys. 2/2018)

Single Cell Analysis

> Automatisation for high throughput image acquisition. > Systems Biology: cell cycle regulatory network of S. meliloti. Outlook > Monitoring the dynamics of proteins implicated in the control of the bacterial cell cycle in individual cells is possible in PDMS chips in face of typical length scales of only 0.5 to 3 microns. > High cell viability visible in the observed division time of about 20...

Single-Cell Repertoire Analysis Demonstrates

Single Cell Isolation and Analysis

Individual cell heterogeneity within a population can be critical to its peculiar function and fate. Subpopulations studies with mixed mutants and wild types may not be as informative regarding which cell responds to which drugs or clinical treatments. Cell to cell differences in RNA transcripts and protein expression can be key to answering questions in cancer, neurobiology, stem cell biology,...

2012 Single Cell Analysis Workshop

Title: Protein Activity in Single Cell Signaling Networks Microelectrophoretic methods can make direct measurements of the activity of normal and oncogenic kinases in single tumor cells. Issues of heterogeneity within cell populations, both in terms of cell type and biochemical behavior, often mandate single-cell studies for accurate determination of kinase activity in tumor cells. Chemical cyt...

Laser-micropipet combination for single-cell analysis.

Due to its potential for exquisite mass detection limits and resolving power, capillary electrophoresis is used for biochemical measurements on single cells; however, accurate measurements of many physiological parameters require sampling strategies that are considerably faster than those presently available. We have developed a laser-based technique to lyse single, adherent, mammalian cells on...